KMID : 0380020100250020179
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Korean Journal of Biotechnology and Bioengineering 2010 Volume.25 No. 2 p.179 ~ p.186
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Optimal Strategy for Ethanol Production in Repeated Fed-batch Operation Using Flocculent Sacchromyces cerevisiae
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Lee Sang-Eun
Yeon Ji-Hyeon Seo Yong-Chang Kang Do-Hyung Lee Hyeon-Yong Jung Kyung-Hwan
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Abstract
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We investigated the optimal strategy for ethanol production using flocculent Sacchromyces cerevisiae ATCC 96581. Considering the characteristic of flocculent yeast, a repeated fed-batch ethanol fermentation was designed, in which non-sterile glucose powder was fed every 12 hours and, after cell flocculation, new feeding medium was exchanged every 24 or 36 hours. We particularly compared this fermentation process with those when cell flocculation was not carried out. Finally, the maximal total ethanol production was 825 g-ethanol during 120 hours, in which the time interval of withdrawal-fill of feeding medium was 24 hours and cell flocculation was carried out.
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KEYWORD
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flocculent yeast, Sacchromyces cerevisiae ATCC 96581, repeated fed-batch culture, ethanol production
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